| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.041643 |
| Chromosome: | chromosome 2 |
| Location: | 1292149 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g082400 | (1 of 2) IPR000104//IPR001394//IPR028889 - Antifreeze protein, type I // Peptidase C19, ubiquitin carboxyl-terminal hydrolase // Ubiquitin specific protease domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTGTGTGGATGTGTGGATGTGCATGTGACGAGGGACCTGGGCGTTCCTC |
| Internal bar code: | ATTCGTGTGTACAATCCAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3858 |
| LEAP-Seq percent confirming: | 42.8571 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCATGCTCTAGTGCTCGAT |
| Suggested primer 2: | GCGATGATCTCAGCAAAGCG |