Insertion junction: CLIP2.041667_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041667
Chromosome:chromosome 2
Location:6549470
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389150 FAP93 Flagellar Associated Protein 93 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGGTGAGGCGCTTGTGCTCGGCGATGAGCAGCGCGCTCACGTCCGCCAGC
Internal bar code:GTCGTCGTACAGGCAGATGTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:3375
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGCTAAACACGGAGCTGA
Suggested primer 2:GAGTCAAGCTCACCCACCTC