| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041670 |
| Chromosome: | chromosome 12 |
| Location: | 1664330 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486551 | PFH19,PHX19 | Putative prolyl 4-hydroxylase; (1 of 1) 1.14.11.7 - Procollagen-proline 3-dioxygenase / Prolyl 3-hydroxylase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACGCAGAGGTTCCGGGCCGTCCTGTAACGTGACATAACGTAAGGTAA |
| Internal bar code: | CTACGTGAGTCTTTCGCTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2701 |
| LEAP-Seq percent confirming: | 90.7407 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCTGCGAGATGTGGGTGA |
| Suggested primer 2: | CACAGCACACTCAACACGTG |