Insertion junction: CLIP2.041677_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.041677
Chromosome:chromosome 7
Location:2923282
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g332700 (1 of 1) PF00069//PF13639 - Protein kinase domain (Pkinase) // Ring finger domain (zf-RING_2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTGACTCGAAACCTTTGCCATCCAACAGTCGGCAGTCGCTGACGCCGAC
Internal bar code:ACTAGCAACAACTGTGTTCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:162
LEAP-Seq percent confirming:7.69231
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GGACACACACACACATGCAC
Suggested primer 2:GCAGCTTGGTGACACTAGGT