Insertion junction: CLIP2.041703_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041703
Chromosome:chromosome 8
Location:1410135
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g364351 FAL12 Protein of unknown function; (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAGGTGGGGTGGATGGGGTTTCGGGGGGGGGGGGTATTGGGCTACCCA
Internal bar code:TTTATCCACCGCGAACTAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1828
LEAP-Seq percent confirming:84.6154
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACACACCT
Suggested primer 2:CCTGTTACAGGCACGTCTGT