Insertion junction: CLIP2.041703_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.041703
Chromosome:chromosome 8
Location:1410136
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g364351 FAL12 Protein of unknown function; (1 of 3) 2.4.1.68 - Glycoprotein 6-alpha-L-fucosyltransferase / Guanosine diphosphofucose-glycoprotein fucosyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCCGCCCGGCCCTCACCTGGATCCCAAACACCGGGGGCGCTGCCAGC
Internal bar code:TTTATCCACCGCGAACTAGAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:785
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGTTACAGGCACGTCTGT
Suggested primer 2:ACACACACACACACACACCT