| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041707 |
| Chromosome: | chromosome 12 |
| Location: | 8406225 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550300 | (1 of 1) PTHR24361//PTHR24361:SF276 - MITOGEN-ACTIVATED KINASE KINASE KINASE // PROTEIN KINASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCACAGCCGCGAGCTCAAGAGCCGGGCCAGGTGGGTGCACTGAGTG |
| Internal bar code: | CGTTTGTTCCATTCTTACTATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 278 |
| LEAP-Seq percent confirming: | 58.0645 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCTCACCCTCATCAGGA |
| Suggested primer 2: | ATCACACTGCTGGAGATGGC |