Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | + |
Strain: | CLIP2.041717 |
Chromosome: | chromosome 13 |
Location: | 442469 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre13.g564850 | (1 of 1) PTHR31651:SF3 - AUXIN EFFLUX CARRIER FAMILY PROTEIN | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAGCCCGCCACTCACCCGCGCTCCAAGCCCCCCACAGCAGCGCCCCGC |
Internal bar code: | GCAATAACGCTCTAGGTCCTTG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1641 |
LEAP-Seq percent confirming: | 92.5926 |
LEAP-Seq n confirming: | 25 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCCGTACATACACCCACAC |
Suggested primer 2: | CGCCATTTGTGATAGGTGCG |