| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041721 |
| Chromosome: | chromosome 11 |
| Location: | 4336385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482650 | EZY3 | Early zygote expressed protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCCAACCCAAGCCCTGCAGCGGGCCGCGGACCGACGACCTCTCCGTCCA |
| Internal bar code: | AGGTCGACATTATGGGGCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2547 |
| LEAP-Seq percent confirming: | 99.0196 |
| LEAP-Seq n confirming: | 101 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 102 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCCGGATTTGATTGCTGAG |
| Suggested primer 2: | GCACAGGGAACAGAAGACGA |