| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041725 |
| Chromosome: | chromosome 12 |
| Location: | 4952463 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g524550 | (1 of 2) PTHR14614//PTHR14614:SF3 - HEPATOCELLULAR CARCINOMA-ASSOCIATED ANTIGEN // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGAATGACACTCCTTGTGAGACTTCACCTTTGCTTCTTCGCTTGACTCTT |
| Internal bar code: | GCGCTAGCCTTACTTATTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1563 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCAACCGTCTTCCTGC |
| Suggested primer 2: | GCTGAAGAACGGAGCAGTCT |