| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041726 |
| Chromosome: | chromosome 12 |
| Location: | 7612773 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g555850 | DESI1,DeSI-1 | DeSI-type SUMO protease; (1 of 2) PTHR12378//PTHR12378:SF7 - UNCHARACTERIZED // DESUMOYLATING ISOPEPTIDASE 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTGAAGACGGCGAGAAGGTGCGCTCCTAGTCATAAGGAAGCGTCACGG |
| Internal bar code: | CTAACATTCTCAAATTTGTGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4644 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 108 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGTGGCTTCCTCGTGATGT |
| Suggested primer 2: | ATCCTATTTGGCCTCTCGCG |