Insertion junction: CLIP2.041759_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041759
Chromosome:chromosome 14
Location:2297086
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g622600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTCCTATACACAATGACAGTAGCGTGGTAGAGGTTCCCACAGCTTTGC
Internal bar code:TTAGGTTGACTAGGAAGCGACC

Confirmation - LEAP-Seq

LEAP-Seq distance:4630
LEAP-Seq percent confirming:46.0784
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:55
LEAP-Seq n unique pos:102

Suggested primers for confirmation by PCR

Suggested primer 1:AAGCGTGGTCAACCCTGTAG
Suggested primer 2:GCTGCTTGGCCTTGAAACTC