| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041766 |
| Chromosome: | chromosome 16 |
| Location: | 7654701 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685277 | AP2M2 | Mu2-Adaptin; (1 of 1) K11826 - AP-2 complex subunit mu-1 (AP2M1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGAGTGGCGCAGGTACATGAACGTGACCGAGCCAAGCGTCCGGATCGG |
| Internal bar code: | ATGGTTGTCATAGTTAAACATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTGCAGGTGACATGTCTG |
| Suggested primer 2: | CGTAGTCCATCACCTCGTCG |