Insertion junction: CLIP2.041766_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.041766
Chromosome:chromosome 16
Location:7654704
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g685277 AP2M2 Mu2-Adaptin; (1 of 1) K11826 - AP-2 complex subunit mu-1 (AP2M1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGACATCTATGTCTTGATGCTCACTCGGAGTAACGGCAACGCTATGCTGT
Internal bar code:TGGAGTGGAACTGCGATACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1711
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGTAGTCCATCACCTCGTCG
Suggested primer 2:AGGTGCAGGTGACATGTCTG