Insertion junction: CLIP2.041770_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041770
Chromosome:chromosome 13
Location:1924028
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g576050 ZIP2,CrZIP10 (1 of 1) PTHR11040:SF61 - PROTEIN ZNTA; Zinc/iron transporter CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCAATGGACACGCGCACGGGCACAGCGGCGCCAGCCTGTCTAGATCTGC
Internal bar code:GACTTAGTTCCATACAAAAAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1388
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:22
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGTGGCTGTAGTTGGGAT
Suggested primer 2:CGCTTCCGCTCATCAATTGG