Insertion junction: CLIP2.041782_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.041782
Chromosome:chromosome 3
Location:3994562
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g171600 XPP1,XPP Exopolyphosphatase; (1 of 2) K03787 - 5'-nucleotidase (surE) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCTCATAATGTACTGAATGGTGCACCCAACCCGCCACCCGCCGCCCCGCC
Internal bar code:TATGAACAGTAGGTTTCTAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:131
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCGAAAGGACACTGGACTCA
Suggested primer 2:GTCTCCTAACCACACGCACA