| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041798 |
| Chromosome: | chromosome 5 |
| Location: | 2746392 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240650 | DNJ32 | (1 of 1) K09506 - DnaJ homolog subfamily A member 5 (DNAJA5); DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTGAACTATGAATGTGACCACCTCTACTACTGTTCCTGGCTGCGGCTTC |
| Internal bar code: | GTTGAATAATATCTTTTCTCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1104 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 14 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGTAGATGTCGGCCCA |
| Suggested primer 2: | GACCACCGCGATCAGATCAT |