| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.041805 |
| Chromosome: | chromosome 16 |
| Location: | 7076106 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g674852 | HAP2,GCS1 | (1 of 1) PF10699 - Male gamete fusion factor (HAP2-GCS1); Generative cell specific-1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAGAGGGAAGCCTGACAAATGATATAAGTGGGGAGCTTGGGTTGTCGA |
| Internal bar code: | GCAAGGCTCGTCAGACGAGCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1108 |
| LEAP-Seq percent confirming: | 98.5294 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTGTCAAATCGTCGTGT |
| Suggested primer 2: | TGCTTCCATGTTTGTGCTGC |