| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041817 |
| Chromosome: | chromosome 16 |
| Location: | 6448805 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g675050 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTCCGTGTGACTCCGCGCACAGTGAGTCCTAGCCAAGCCTCAACCCGCC |
| Internal bar code: | TTAGCGAGTCACGTGTAATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4194 |
| LEAP-Seq percent confirming: | 65.6489 |
| LEAP-Seq n confirming: | 86 |
| LEAP-Seq n nonconfirming: | 45 |
| LEAP-Seq n unique pos: | 131 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGTTGCATGCACGCATAT |
| Suggested primer 2: | GTGGACTCAACTCACCCAGG |