| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041819 |
| Chromosome: | chromosome 10 |
| Location: | 2770207 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438300 | LTH4 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease; LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCAAAGAAGTAGCAGAAGGTGAAGGTGGTCCACACGATGCGCGCCGTTC |
| Internal bar code: | AAACCGGGTCAACGCAATTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4623 |
| LEAP-Seq percent confirming: | 82.2581 |
| LEAP-Seq n confirming: | 51 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGCCGGGAGACTAATTCG |
| Suggested primer 2: | ACGCACAATGGGTAGAAGGG |