| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041821 |
| Chromosome: | chromosome 7 |
| Location: | 130032 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g313000 | (1 of 2) 2.7.7.48 - RNA-directed RNA polymerase / RNA nucleotidyltransferase (RNA-directed) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGCGGCGAGGGGCGCCATGGCCGCACACACGGCGTTGCGCAGGCGAGC |
| Internal bar code: | AAAGCTAGCTAAATCACGTTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1568 |
| LEAP-Seq percent confirming: | 70.8333 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 48 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGCTCCAAACGCAATGCA |
| Suggested primer 2: | TGCCCACATCCTACACACAC |