| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041823 |
| Chromosome: | chromosome 11 |
| Location: | 4307427 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g482483 | (1 of 1) K12599 - antiviral helicase SKI2 (SKI2, SKIV2L) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTATTTCAATGGAGGGCGGCGGCCAGGGCAGTGGGCCGATGAAACGTCG |
| Internal bar code: | ACTCTTTGATTACGCTTGATCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 111 |
| LEAP-Seq percent confirming: | 87.5 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGTTGTAAGTACCAGCC |
| Suggested primer 2: | CACATCTCGGATCAGGTCGG |