Insertion junction: CLIP2.041839_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.041839
Chromosome:chromosome 16
Location:1433256
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652400 FAL18,FAP183 (1 of 17) PF13202 - EF hand (EF-hand_5); EF-hand domain Flagellar Associated Protein 183 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCTGACGCGGTTGCTGACGCCGGCTGCTGCAGGTTGAGGAGGTGGA
Internal bar code:GCCTAATACTGGTTGGACGAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1264
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:CACTGTCAGCCGGTACTTGT
Suggested primer 2:CACAAGGGTGTTTGCTTCCG