| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041873 |
| Chromosome: | chromosome 1 |
| Location: | 3853480 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025350 | FAP235 | (1 of 3) PF03703 - Bacterial PH domain (bPH_2); Flagellar Associated Protein 235 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATATGTCGGCATGTGTCAGCTGCGCAAGCTGGCGCACCCTCCAGGACAG |
| Internal bar code: | GGGTATAATGGGATTAGCTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1797 |
| LEAP-Seq percent confirming: | 3.25203 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 119 |
| LEAP-Seq n unique pos: | 123 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTGCATGACAGCGTAATG |
| Suggested primer 2: | ACTGTGGACGGCTTACTGTG |