| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.041883 |
| Chromosome: | plastome |
| Location: | 203657 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802336 | orf854 | Wendy transposon | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAGGCACGGGGATGGTAGTACTACCATCCTTGCCGCCGTAGCGGAGCCC |
| Internal bar code: | ATATGTGAGGGTGATGCAGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1417 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 26 |
| LEAP-Seq n unique pos: | 91 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCGGAGCGGATGGAAAAA |
| Suggested primer 2: | CTCTCCAACTTGGCGGCTAA |