Insertion junction: CLIP2.041900_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.041900
Chromosome:chromosome 6
Location:3493071
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278111 (1 of 6) IPR000595//IPR005821//IPR018490 - Cyclic nucleotide-binding domain // Ion transport domain // Cyclic nucleotide-binding-like CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCGCGCAGGTGTGAGGGCGCACACACACGACCCGCTGGGGCTGCGTGG
Internal bar code:CACTAGGATCATTAGAGACTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:1337
LEAP-Seq percent confirming:97.8723
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:47

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGGTCATGGTGGTGTAGG
Suggested primer 2:TGTCTCTGCTGGAGCTGTTG