Insertion junction: CLIP2.041915_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.041915
Chromosome:chromosome 7
Location:1343339
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g322550 FAP240 Flagellar Associated Protein 240; (1 of 1) PF14977 - FAM194 protein (FAM194) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTATCGGTCTTGCAGCAGGTTGCCCTCGCTCGCGTCTACTTTCCACATC
Internal bar code:AAAGGGGTGATTGGTAGGTGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:276
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTCAGAAATGCACATCCG
Suggested primer 2:TACATCGCGCCATGACTACC