| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041929 |
| Chromosome: | chromosome 9 |
| Location: | 1197735 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g400950 | NCT2 | (1 of 2) PTHR11662:SF3 - INNER MEMBRANE TRANSPORT PROTEIN RHMT; High-affinity nicotinic acid transporter | 5'UTR |
| Cre09.g401000 | SNE3 | UDP-N-acetylglucosamine/UDP-glucose 4-epimerase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTGCCGGAATGGACCAGGGCATATCCAGCGGCGGCGCCAGCGGCCGC |
| Internal bar code: | TCGGTACCGGTTCGTTAGTGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2226 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGACAGCATGCAAACTGC |
| Suggested primer 2: | TCCGATGGAAACCGTGCTAG |