| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041933 |
| Chromosome: | chromosome 2 |
| Location: | 1477827 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083850 | (1 of 173) IPR029058 - Alpha/Beta hydrolase fold | 5'UTR | |
| Cre02.g083900 | EXN5 | (1 of 1) PTHR13620:SF15 - EXONUCLEASE MUT-7 HOMOLOG-RELATED; 3'-5' exonuclease | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGAAAGAGAAACGCCGAAACCCCACGCATTGTCCAGGTCAACGAATTC |
| Internal bar code: | CTCTTAAATCTTCAGGTAGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATGAACCATGTGCTGCGAG |
| Suggested primer 2: | CACGCGTGTGTTGGTGTATG |