| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041943 |
| Chromosome: | chromosome 2 |
| Location: | 5708201 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g116300 | (1 of 1) K15171 - transcription elongation factor SPT4 (SUPT4H1, SPT4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCGCCAAGCCTCAGGCCCCCGGGGCGCTCGGCTGTGTGTGTGCGCC |
| Internal bar code: | ATAACAAGAACTGGTTCAGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1581 |
| LEAP-Seq percent confirming: | 96.2963 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAATACGCTGCTGTGAACG |
| Suggested primer 2: | AGGGAAGTTACAGCCAAGCC |