| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041946 |
| Chromosome: | chromosome 8 |
| Location: | 3886432 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g381900 | (1 of 2) PTHR22763:SF71 - PROTEIN Y105C5B.11, ISOFORM B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTTCCACGTGTACTACAAGGGTAGGTGGTGCAAGGTGGTTGCTTAAG |
| Internal bar code: | CTCACGCGCCTATGTTTAGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1178 |
| LEAP-Seq percent confirming: | 57.5758 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTGCTGCTACTGCTACTG |
| Suggested primer 2: | CGGTAGTGACGATGACGGAG |