| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.041951 |
| Chromosome: | chromosome 12 |
| Location: | 2196229 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509100 | FAP307 | Flagellar Associated Protein 307 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGGGTTTTGCCCCCCGGCTTACATGACCAGGCGTTTCAGGGCGAATCAG |
| Internal bar code: | TGGTGTGTTGCAAGAGTAGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6066 |
| LEAP-Seq percent confirming: | 95.6989 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCACCCAAACTACTGTCGA |
| Suggested primer 2: | GTGAATCCCCTACGTAGGCG |