| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.041957 |
| Chromosome: | chromosome 4 |
| Location: | 2109253 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g219725 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCCAAGCGGGCTGTGGCTCCAAAGCTGTCTCACTCACCCGGTCCCGT |
| Internal bar code: | TTGAGGACCGGTGATAGCTCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1606 |
| LEAP-Seq percent confirming: | 90.3226 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGGAAGCCTCGTACACG |
| Suggested primer 2: | GTCCTTCGTCCCTAAGCACC |