| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.041974 |
| Chromosome: | chromosome 5 |
| Location: | 2452873 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g800559 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGATGGCACCCCTTCGTCTTCGTGCCTGAAGAATTTGCGTCTCAAATG |
| Internal bar code: | CACTCAAGTGTGAGGTCCAGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 408 |
| LEAP-Seq percent confirming: | 69.2308 |
| LEAP-Seq n confirming: | 9 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGGTTCGGTTTAGGTTGGG |
| Suggested primer 2: | CTGTAGACCAGTGTGCAGCA |