Insertion junction: CLIP2.041982_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.041982
Chromosome:chromosome 12
Location:9222765
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g542900 (1 of 112) 2.7.11.25 - Mitogen-activated protein kinase kinase kinase / MLTK CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTCCCCCCGTCTAGGCGCGGCTCCACGCTGCCGCACACTAGCGGCTT
Internal bar code:TGGGAGAGGCCCCCCCGCCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:2491
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:74
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:74

Suggested primers for confirmation by PCR

Suggested primer 1:TACGGCCACCATAATCGACG
Suggested primer 2:CGCCACAACAAGAACAAGGG