| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.041993 |
| Chromosome: | chromosome 5 |
| Location: | 275385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243351 | (1 of 3) IPR000477//IPR002156//IPR012337 - Reverse transcriptase domain // Ribonuclease H domain // Ribonuclease H-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACACTACGTTGAGGTCTACGGCGATGCAGCGGCGCACTCCGTCCCAG |
| Internal bar code: | GTTTATGCTCGTTTTCACTACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2826 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTTATGGACTCTCTGGGCG |
| Suggested primer 2: | CTTCATGCACAGCACCAACC |