Insertion junction: CLIP2.041995_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.041995
Chromosome:chromosome 12
Location:399684
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g495350 POC12 (1 of 1) K19332 - Meckel syndrome type 1 protein (MKS1); Proteome of centriole protein 12 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACCTCACAGTCACACGCGCCGCGCGAGTCAGCAGGGCCACTGCGGGAGA
Internal bar code:TGGAGTAGTAGCGGGACCTACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3463
LEAP-Seq percent confirming:84.127
LEAP-Seq n confirming:53
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTATGGACCCGGGTTCAT
Suggested primer 2:CTCAAGGAGTGTGTGACGCT