Insertion junction: CLIP2.041996_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.041996
Chromosome:chromosome 4
Location:2260737
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g220500 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGATGTGATATCCGCCATCGCCTCATCCTTGGTGTCTGCCGTGACGCAG
Internal bar code:TCGTTTACTGCCAGGCCGAAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:964
LEAP-Seq percent confirming:75.2809
LEAP-Seq n confirming:67
LEAP-Seq n nonconfirming:22
LEAP-Seq n unique pos:89

Suggested primers for confirmation by PCR

Suggested primer 1:CCTGGACACCTTCGACTTCC
Suggested primer 2:GTCGTGCTCACCACCTTGTA