| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042006 |
| Chromosome: | chromosome 13 |
| Location: | 1841227 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g575250 | (1 of 1) PTHR10663:SF114 - PROTEIN MON2 HOMOLOG | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGTGCCGTTGCGGCGGCGGGCGGCGGCGCCAACGGTCCACCGCTGGCA |
| Internal bar code: | TCGCGTTGGGATTTTTGACTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2170 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGTGTTCACAGGTTGCG |
| Suggested primer 2: | CAGACAGCACTCACAGCAGA |