Insertion junction: CLIP2.042020_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.042020
Chromosome:chromosome 1
Location:7473332
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g052650 APT2,VIP2 inositol hexakisphosphate kinase; (1 of 2) 2.7.4.21 - Inositol-hexakisphosphate kinase / IP6K intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACACACACACATTCCACCCCAACCCCTAAACCCACCCCCCCAGGCG
Internal bar code:CTGCGTTGCCTTGAAGGCAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:365
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CACACACACACACAGAAGCG
Suggested primer 2:CGAGGACCACAACATCCACA