| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042024 |
| Chromosome: | chromosome 10 |
| Location: | 3262070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g442750 | (1 of 1) K19045 - E3 ubiquitin-protein ligase BIG BROTHER and related proteins [EC:6.3.2.19] (BB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGGCGCCGTTTGCATTTAGCTTCTTAGGGCATCGCCAATCATGTCACT |
| Internal bar code: | TGAGAAAGGTCGCGGAATTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5125 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 116 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 116 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTGTACGTATGCAAGCAG |
| Suggested primer 2: | GAGGAAACAGCACACGAGGA |