Insertion junction: CLIP2.042032_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042032
Chromosome:chromosome 8
Location:1635530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g365825 (1 of 1) 1.14.99.42 - Zeaxanthin 7,8-dioxygenase / Zeaxanthin 7,8(7',8')-cleavage dioxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAGGCAGCGGGCGGCGCGCAGGCGCAGTCGCTGCCGAACCTGGTGTCG
Internal bar code:GAGACTTATTAGGCGAATAAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1375
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:42
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCGTGAATTTGGCTGTTG
Suggested primer 2:ATGCGCGACGAGTCATACAT