| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042049 |
| Chromosome: | chromosome 10 |
| Location: | 1263715 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g426700 | CYP739A4,CYP28 | (1 of 1) 1.14.13.109 - Abieta-7,13-dien-18-ol hydroxylase / CYP720B1; Cytochrome P450, CYP197 superfamily | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCCTCCTGCACGCGCGTGCTCATGGCGACCATGGCCCAGAACGTGAACA |
| Internal bar code: | AAAATCGTTACGAGGCACTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 57.4468 |
| LEAP-Seq n confirming: | 27 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 47 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCTTCCAACACCCCACCTC |
| Suggested primer 2: | TACGATGGATGGCTCGAACG |