Insertion junction: CLIP2.042058_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.042058
Chromosome:chromosome 6
Location:6316530
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g292900 (1 of 10) PTHR12270:SF6 - GLYCOSYLTRANSFERASE-LIKE PROTEIN LARGE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCGTTTGGGCTGGTGTTTACAACTCCCCCCACGCGCGCGCACACGCGCG
Internal bar code:TTGAGTAATCCGCTGGCACCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:442
LEAP-Seq percent confirming:63.6364
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GGGGTTGTTATCGGTGGAGG
Suggested primer 2:CCATTAGGTCCACGGCACAT