Insertion junction: CLIP2.042075_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.042075
Chromosome:chromosome 11
Location:903070
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467653 (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGGAATCCCGTGCGTTAACAGACTTTTACCGCCGTTTTGCCTACAACTGC
Internal bar code:ATGCCAAGCAGTGTTCCTGTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2009
LEAP-Seq percent confirming:78.2609
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:10
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:TACCTCTTCCCCTTCCTCCG
Suggested primer 2:TGTACACCGCATCACACGAA