| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.042083 |
| Chromosome: | chromosome 8 |
| Location: | 4171314 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383650 | NUP85 | Nucleoporin 85; (1 of 1) K14304 - nuclear pore complex protein Nup85 (NUP85) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACCACCCCGCAGGATTTTGTGTCGTGGCTGGCAGTGCACGGCGACGCG |
| Internal bar code: | CCGAGATAGCTATGTAAGTAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 53.3333 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAACACTGCACACACACACC |
| Suggested primer 2: | GGTCGTACTGCTGCATCGTA |