| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.042095 |
| Chromosome: | chromosome 15 |
| Location: | 546956 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g636176 | (1 of 10) IPR000104//IPR001810 - Antifreeze protein, type I // F-box domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGGAAGTCGTGTCCGAACACGTGGCCCCCCACCGCCTGTGGGTTGCAA |
| Internal bar code: | CCTCTGAGCTTTGTATATTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2315 |
| LEAP-Seq percent confirming: | 78.3333 |
| LEAP-Seq n confirming: | 47 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGACCTGCCAGATCACTT |
| Suggested primer 2: | CCGCTCGTTTTCGTTGGTTT |