Insertion junction: CLIP2.042107_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.042107
Chromosome:chromosome 9
Location:2622109
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g801018 (1 of 1) PTHR11390//PTHR11390:SF21 - PROKARYOTIC DNA TOPOISOMERASE // DNA TOPOISOMERASE 3-ALPHA intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGCACCCCCCACACAAACATCCACCCCCATCCCCAACCCCCACCCAA
Internal bar code:GAGTGTGACACTCGTAATGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:270
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GAGCTCGCGCATACAAAAGG
Suggested primer 2:ATGAAGGGGTTAGTGGCAGC