| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042117 |
| Chromosome: | chromosome 6 |
| Location: | 7958723 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g304400 | SOUL4 | SOUL heme-binding protein; (1 of 2) PTHR11220:SF35 - SOUL HEME-BINDING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACCACACCCGTGTTCTCAGACACGGCCGGCAGCATGCGCTTCGTGAT |
| Internal bar code: | GCGCTGTCCTGGAGGAGTGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1753 |
| LEAP-Seq percent confirming: | 74.0741 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAGCTCCAAACTCGGACAC |
| Suggested primer 2: | TACGGGGCGAGGGGATATAG |