| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.042129 |
| Chromosome: | chromosome 12 |
| Location: | 3044595 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g801387 | (1 of 1) K17867 - diphthamide biosynthesis protein 4 (DPH4, DNAJC24) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGAGGTCCCAAGCCTCCAAGCCCAGCGCCAAAGGCACCCAGGCCTATG |
| Internal bar code: | TGATGATGTACTTAACCTAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3754 |
| LEAP-Seq percent confirming: | 90.1961 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCAGCGCACCCATTATG |
| Suggested primer 2: | AATGAGCACAGTCTGACGCA |